By reverse transcription-quantitative PCR (RT-qPCR), we found that LncRNA NDRG1 was up-regulated significantly in osteosarcoma cell lines (MG63, U2OS, HOS and 143B) and tissues, and functional experiments including CCK8, EdU, transwell assay and wound healing assay showed that LncRNA NDRG1 aggravated proliferation and migration of osteosarcoma in vitro. The gene discussed is NDRG1; the disease is osteosarcoma.