Revealed 24 significant mutant genes (SMGs) identified in single cell(SORD,REXO2,REC8,PTCH2,CTAGE5,RNF20,RBBP4,MGAT4A,KIF15,XYLT1,IGF2BP3,DLC1,TG,CDC27,BAZ1A,ETV6,FLG,NEK6,NSD1,PDE4DIP,RAF1,RNF2,SMO,ZNF483).The mutant genes CDC27 and FLG might alter the protein conformation only in single cell but not in the corresponding tumor tissue. This evidence concerns the gene NEK6 and neoplasm.