Considering that the significant difference in tumor volume of mice between the anti-PD-L1 monotherapy and the combinatory therapy could affect the immunological study, we isolated tumor tissues in the similar tumor volume ~1000 mm3 in the different time: DMSO group on day 12, ART or anti-PD-L1 monotherapy group on day 15, and the combination therapy group on day 21. Here, CD274 is linked to neoplasm.