The results indicated that baicalein exerting its protective effects against NAFLD was closely related to steroid biosynthesis, fatty acid degradation, the PPAR signaling pathway, peroxisome, tryptophan metabolism, ABC transporters, propanoate metabolism, valine, leucine and isoleucine degradation, 2-Oxocarboxylic acid metabolism, fatty acid metabolism, complement and coagulation cascades, ECM–receptor interaction, protein digestion and absorption, etc. This evidence concerns the gene ABCG2 and metabolic dysfunction-associated steatotic liver disease.