Furthermore, ROCK1-overexpressing mice are characterized by more severe cardiac fibrosis and upregulation of TGFβ1, and the administration of fasudil to rats with T2DM induced by high-fat diet-feeding in combination with STZ reduces the cardiac deposition of type I and type III collagen, and the mRNA expression of JNK and TGFβ1 (136). Here, TGFB1 is linked to fibrosis.