Therefore, expression of the vascular endothelial marker CD31 was detected via IHC, and the results showed that angiogenesis significantly decreased in the Aged group (Aged vs Control: 95% CI, -26.68 to -11.10; P<0.0001) whereas it significantly increased after treatment (Aged+CF vs Aged: 95% CI, 9.55 to 26.51; P<0.0001), suggesting that CEFFE could promote ovarian medulla angiogenesis in aged mice (Figure 3A). Here, PECAM1 is linked to cystic fibrosis.