A total of 65,535 cells were divided into 7 cell clusters, a total of 6,539 epithelial cells from ccRCC multiple regions (Near, Far, Center, and Lymph node) and normal tissues of 6 ICB-treated and untreated patients were extracted, and 6 ccRCC cell clusters were identified based on AQP3, GPX3, CCNI, STMN1, VCAM1, and VCAN expression (Figures 1B, C; Figures S1C, D; Table S5). This evidence concerns the gene VCAN and nonpapillary renal cell carcinoma.