Comparing the entry efficiency of these mutant pseudoviruses into HeLa Mouse-ACE2 cells to WT D614G, we found 7 single substitutions (R408S, K417N, Q493R, Q493K, G496S, Q498R, and N501Y highlighted in dark red) substantially improved while 3 (S371L, S371F, and S375F in dark blue) decreased infection (Figure 5A). The gene discussed is ACE2; the disease is infection.