AKT1 and osteosarcoma: In osteosarcoma cell lines (MG-63 and U2OS), MIR4435-2HG can up-regulate the protein levels of p-PI3K and p-AKT, suggesting that MIR4435-2HG may activate the PI3K/AKT pathway to promote the growth of osteosarcoma cells, Invasion, migration and apoptosis (46).