C57BL/6 mice were administered 5 × 105 melanoma B16-GP or 5 × 105 B16-OVA i.v. On Day 8, when lung metastasis was established as evidenced by luciferase reporter assay (Supplementary Figure 1A), 2 × 106 activated SMARTA CD4+ T cells in total (one dose or divided into three doses for successive 3 days) were transferred into B16-GP tumor-bearing mice i.v. by tail vein injection. The gene discussed is CD4; the disease is melanoma.