Furthermore, a randomized clinical trial in patients after major abdominal surgery with an increased AKI risk demonstrated that early prediction of PO-AKI by urine TIMP-2 × IGFBP7 followed by implementation of KDIGO care bundle would reduce PO-AKI severity, post-operative creatinine increase, length of ICU and hospital stay in patients after major non-cardiac surgery (34). This evidence concerns the gene IGFBP7 and acute kidney injury.