According to Figures 5B,C, TGF beta signaling route, Wnt signaling pathway, Melanoma, Glioma, Cancer pathways, Focal adhesion, JAK STAT signaling pathway, T cell receptor signaling pathway, B cell receptor signaling pathway, Mark signaling pathway were prominent in NMRG Cluster C. Furthermore, the intricacy of immune cell infiltration among the three species subtypes was revealed by ssGSEA enrichment analysis. This evidence concerns the gene TBXT and melanoma.