In total four different types of AD in vitro models were used: (1) primary cell cultures isolated from wild-type (WT) rat or mouse brain tissue where neurons or astrocytes are treated with Aβ to induce an AD-like phenotype, (2) primary cell cultures isolated from an AD animal model, (3) control human embryonic stem-cell derived or human induced pluripotent stem cell (iPSC)-derived neuron-astrocyte co-cultures treated with ApoE protein, and (4) human iPSCs with different ApoE genotypes. This evidence concerns the gene APOE and Alzheimer disease.