Consistent with the development of steatosis in the HFD group, important upstream regulators involved in lipid catabolism (peroxisomal acyl-CoA oxidase 1 (ACOX1), AMP kinase (AMPK) and mitochondrial biogenesis (PPARGC1a) were all inactivated with HFD (Table 3). This evidence concerns the gene ACOX1 and steatosis.