Studies on the correlation between glycosylation and the regulation of immune response (Li et al., 2018), PD-1 receptor pathway (Liu et al., 2020b), and tumor microenvironment (Chandler et al., 2019) suggest that new tumor therapeutic targets may be found by paying attention to glycosylation, so we planned to construct stable cell lines to continue to study deeper mechanisms and explore the possible pathways of ALG3 affecting hepatocellular carcinoma. The gene discussed is ALG3; the disease is hepatocellular carcinoma.