The authors performed single-cell analysis of microglia from four different postmortem brain regions including frontal lobe, temporal lobe, thalamus and subventricular zone of medicated individuals with MDD and they found no evidence for the induction of canonical pro-inflammatory (IL-1β, IL-6, and TNF-α) and anti-inflammatory cytokines such as IL-10 (98). Here, TNF is linked to major depressive disorder.