UCN3 and Pruritus: We found that 13.0% (6/46) of Ucn3+ neurons were excited by Aβ stimulation in the vehicle (EtOH)-treated control group, while 86.5% (32/37) of Ucn3+ neurons were excited by Aβ stimulation in the calcipotriol-treated group (p < 0.0001, chi-squared test, Figure 1B), indicating that the mechanical itch microcircuit in the spinal cord is sensitized under chronic itch conditions.