P. chabaudi schizonts are sequestered during the infection in mice (Gilks et al., 1990; Brugat et al., 2014), and can only be obtained after a period of in vitro culture, which may affect the detection of PIR at that stage and improving the culture of P. chabaudi would clarify PIR expression at this stage of parasite development. The gene discussed is PIR; the disease is infection.