Through analyzing differentially spliced mRNA transcripts upon RBM17 knockdown in K562 cells, we also noted that many splicing events affected by RBM17 knockdown have previously been found abnormally spliced in secondary AML (sAML) LSCs compared to aged normal hematopoietic progenitor cells (HPCs)15, including transcription related factors TAF6, NFE2, TBL1XR1, CNOT2, MGA, COMMD3, SUPT5H, and RNA processing proteins RBM39, SF1 and EIF4H. The gene discussed is CNOT2; the disease is acute myeloid leukemia.