In the present study, we demonstrated that FXR overexpression significantly increased the myocardial retention rate of engrafted ADSC and improved the cardioprotective effects of ADSC against ischemic heart injury, as evidenced by improved cardiac function, reduced myocardial infarct size, increased survival rate, enhanced angiogenesis, and reduced cardiomyocyte apoptosis. The gene discussed is NR1H4; the disease is myocardial infarction.