Genes related to growth factor binding, extracellular structure/matrix organization, tissue and cell development, HCC, Wnt signaling pathway, and Hippo signaling pathway were enriched in clusters 1, 2, and 3, whereas genes related to positive regulation of defense response, cell chemotaxis, and IL-17, chemokine, and TNF signaling pathways were enriched in clusters 4 and 5 (Fig. 4C and Fig. S6). This evidence concerns the gene TNF and hepatocellular carcinoma.