Consistent with the cellular localization of CC1, GSDMD was also localized in NeuN-positive neurons and Iba-1-positive microglia of cortex and hippocampus (Fig. 3f-g, Supplementary Figs. 1c, f and 4), supporting the occurrence of inflammasome-mediating pyroptosis in cortical and hippocampal neurons and microglia of the IS-induced migraine mouse model. This evidence concerns the gene GSDMD and migraine disorder.