TARDBP and amyotrophic lateral sclerosis: To discover RBPs with increased insolubility in a human ALS model, we applied a well-established dual-SMAD inhibition-based protocol [21, 41, 42, 44] to generate iPSC-MN from six control (Ctrl) iPSC lines, from four iPSC lines originating from two sALS patients, and from two iPSC lines originating from fALS patients with pathogenic variants in the TARDBP gene (Online Resource Table 1; Online Resource Fig. 1a).