NfL is a promising diagnostic and prognostic fluid biomarker with high-clinical value in many neurological disorders.[22–24] Owing to its convenience and lesser invasiveness in clinical practice, blood NfL measurement has been an exciting and active field of research.[25] In this study, we confirmed that pNfL may be used as a biomarker to predict the severity of neuroaxonal injury in patients with AIS. The gene discussed is NEFL; the disease is nervous system disorder.