FGL1 and hepatocellular carcinoma: A quantitative comparison, performed using the gray analysis of the western blot, revealed a lower FGL1 expression in the HCC-LM3, SK-Hep1, SMMC-7721, C3A, HepaG2, Huh7, and Hep3B cells, compared to the normal liver cell line (LO2) (Fig. 2A).