After coculturing with PBMCs, FCA showed that, unlike the results of the gastric cancer cell line cocultured with PBMCs, the percentage of cytokines (TNFαhigh and IFNγhigh) in the shNAMPT group was not significantly different from that in the ShControl group, but PD1, which represents the exhaustion of CD8+ T cells, was significantly decreased compared with that in the control group (Fig. 7d, e). This evidence concerns the gene CD8A and gastric cancer.