Seven of the genes were downregulated by obesity and reversed by VSG specific weight loss including Ido1, Aldoc, Tmem125, Dgki, Slc7a4, Msc, and Ephb3, while four were inversely regulated with obesity elevating Klhl5, Nek6, Arhgap20, and Hp. This evidence concerns the gene KLHL5 and obesity due to melanocortin 4 receptor deficiency.