Further Multivariate Cox regression analyses suggested that ANKRD49 expression, TNM stage, lymph node metastasis, and differentiation were independent prognostic indicators for the overall survival of LUAD patients, indicating that ANKRD49 may be used as an independent predictor of prognosis evaluation for LUAD development (Table 2). Here, ANKRD49 is linked to metastatic malignant neoplasm in the lymph nodes.