In addition, the apoptotic markers, such as caspase-3 and caspase-8, were found to be significantly upregulated in NASH and NAFL mice; and inhibition of either can reduce apoptosis and inflammatory infiltration, which alleviates liver damage in NASH and NAFL mice (Hatting et al., 2013; Wang et al., 2017; Li et al., 2018; El-Derany and El-Demerdash, 2020). This evidence concerns the gene CASP8 and non-alcoholic fatty liver.