Based on previous informatics data, as shown in Figures 2E, 3B, AKT1 was one of the key targets in the top 10 bioenrichments, and Pathways in cancer was the most enriched signaling pathway; therefore, wedecided to test whether mTOR/PI3K/AKT pathway involved in the anti-tumor effect of ASP. The gene discussed is AKT1; the disease is cancer.