In view that diet-induced obese individuals are not always fed with high-fat food and a normal diet is usually preferred when one is planning to lose weight, to further investigate whether the iPRTi strategy is capable practically for obesity management, we also conducted the DIOed group experiment with the diet-induced mice administrated with NC and ob recombinant yeast (Figure 2C). Here, LEP is linked to obesity due to melanocortin 4 receptor deficiency.