TNF and American trypanosomiasis: The genes comprising the selected clusters were functionally annotated, which allowed the identification of many immunological pathways such as tumor necrosis factor (TNF) signaling pathway, NF-kappa B signaling pathway, cytokine–cytokine receptor interaction, Chagas disease (American trypanosomiasis), MAPK signaling pathway, toll-like receptor signaling pathway, IL-10 signaling pathway, and TH17/IL-17 signaling pathway.