We have verified through immunohistochemical analysis that the expression of COL8A1 in GC tissues is distinctly higher than that in normal tissues, and COL8A1 is significantly related to pathological T staging and lymph node metastasis, which suggests that COL8A1 expression may play a part in the progression of gastric cancer. Here, COL8A1 is linked to metastatic malignant neoplasm in the lymph nodes.