Previous studies have established streptozotocin (STZ) induction, Otsuka-Long-Evans-Tokushima-Fatty (OLETF) rats, Akita mice, OVE26 mice, BTBR ob/ob mice, and endothelial nitric oxide synthase (eNOS)-/- mice as different diabetes models and Sirt1 global, podocyte-specific, and proximal tubule-specific overexpression and knockout mice as Sirtuin targeting animal models, and constructed a series of Sirtuin family targeting reagents represented by Resveratrol, and achieved some results as follows. The gene discussed is NOS3; the disease is diabetes mellitus.