Furthermore, in 3D tumor spheroid invasion assays, we observed that YAP1 overexpression induced the formation of pseudopod-like structures on the rim of MCF7 cell spheres (Fig. S1E), whereas YAP1 knockdown significantly inhibited MDA-MB-231 cell growth and invasiveness in the 3D culture environment (Fig. S1F). This evidence concerns the gene YAP1 and neoplasm.