METTL3 and familial pancreatic carcinoma: Furthermore, we performed siRNA experiments to confirm whether PLK1 expression is regulated by METTL3 in another pancreatic cancer cell line (AsPC-1), and confirmed that PLK1 expression is downregulated by METTL3-KD in AsPC-1 as in MIAPaCa-2 (Supplementary Fig. 1d,e).These findings indicate that PLK1 is an important gene regulated via RNA methylation by METTL3, suggesting that it is responsible for various treatment resistances in pancreatic adenocarcinoma because we revealed that control cells were more resistant to chemotherapy and radiotherapy than METTL3-KD cells7.