Since our RNA-Seq experiments identified the regulation of β-catenin/TCF-LEF1 pathway by riluzole in pancreatic cancer, we examined the effects of riluzole on the expression of components of this pathway by q-RT-PCR and TCF-LEF1 transcriptional activity by luciferase assay (Fig. 3). The gene discussed is LEF1; the disease is familial pancreatic carcinoma.