In T‐47D‐implant (human breast cancer cells) tumor tissues expressing high levels of estrogen and progesterone receptors, the probes were able to effectively distinguish tissues expressing estrogen (with SQ650 as the primary signal), progesterone (with ICG as the primary signal), or both estrogen and progesterone receptors concurrently, which was consistent with immunohistochemical results. Here, PGR is linked to breast carcinoma.