However, chemogenetic inhibition of LHHcrt cells did not reduce anxiety after SDS (Fig. S30), and orexin receptor antagonists did not restore the anxiolytic effects that were missing in VTASst-lesioned mice that had undergone SDS (Fig. S31), suggesting that orexin/Hcrt cell inhibition is not required for the anxiolytic actions of VTAVgat-Sst cells. This evidence concerns the gene HCRT and Anxiety.