Although a different analysis, simply comparing the effect of obesity (BMI > 30) on the levels of INSL3, T, cFT, and SHBG for the phase 2 subjects also indicated a significant impact of obesity on INSL3, as well as on the other parameters (Figure S3); the impact is relatively smaller (‐9.4%) for the former than T (‐21.5%), cFT (‐14.5%), and SHBG (‐17.4%). This evidence concerns the gene SHBG and obesity due to melanocortin 4 receptor deficiency.