NES and Cerebral ischemia: Therefore, we used BrdU/Nestin to evaluate DG cellular proliferative ability at day 14 post-ME, and the immunofluorescence staining results presented that BrdU/Nestin expression in the DG began to increase after ME, which denoted an activated proliferative characteristic of NSC as a response to cerebral ischemia, and PNS 120 mg/kg administration remarkedly advanced BrdU/Nestin expression, suggesting that PNS could augment the number of NSC populations in the hippocampus.