As illustrated in Figures 5A,B,D, in sham group, almost few BrdU/Nestin and BrdU/DCX were found in DG, however, both the numbers of BrdU/Nestin and BrdU/DCX were remarkably increased in damaged DG zone of ME rats, demonstrating that the proliferation and migration of NSC/NPC were triggered in response to cerebral ischemia. The gene discussed is NES; the disease is brain ischemia.