For one standard deviation increase in genetically predicted GDF-15 levels, the odds ratio (OR) of gallstones was 1.14 (95% confidence interval [95% CI]: 1.01, 1.29; p = 0.028) in UK Biobank, 1.08 (95% CI: 1.02, 1.14; p = 0.012) in FinnGen, and 1.09 (95% CI: 1.03, 1.15; p = 0.001) in the meta-analysis of the two datasets. This evidence concerns the gene GDF15 and gallstones.