Figures 5A–F indicate the morphological changes of p53-mutant H1437 cancer cells. Following AO/EB staining, untreated control cells (A) and 0.25% DMSO (B) and 0.25% H2O treated (C) cells displayed intact nuclei (red arrows), while lung cancer cells exposed to curcumin (D) and D. calcarata extracts (E-F) contained many cells demonstrating early apoptosis, which fluoresced green/yellow (yellow arrows) and reduced cell numbers. The gene discussed is TP53; the disease is lung carcinoma.