CD274 and cancer: For further analyses, we selected well-known immune checkpoint molecules: PD-1, PD-L1, lymphocyte activating 3 (LAG3), hepatitis A virus cellular receptor 2 (TIM-3), CTLA-4, B and T lymphocyte associated (BTLA), and selectin P ligand (SELPLG), and we found they exhibited opposite prognostic effects in PPRC and PNRC subtypes (Figure 5(b)), implying possible linkage between pyroptosis and checkpoint molecules in different cancer subtypes.