Considering that ASK1 and MAPKs are downstream molecules of TLR4 signal pathway, and the lipid output but not its input is declined in MCD-induced NASH, we speculate that MFG-E8 does not directly regulate lipid metabolism but might block inflammatory cascade-worsened metabolic disorders by inhibiting innate immune TLR4 signal. Here, TLR4 is linked to metabolic disease.