In the current study, overactivated TLR4/NF-κB signaling pathway, as well as the elevated levels of inflammatory mediators, was observed in the MCD-KO group, suggesting that the effect of MFG-E8 in NASH might be mediated by TLR4/NF-κB signal pathway. Here, NFKB1 is linked to metabolic dysfunction-associated steatohepatitis.