In the study of liver cancer, it was found that the full-length apoptosis-inducing factor mitochondrial-associated 1 (AIFM1) (∼67 kDa) was cleaved at its N-terminus to produce truncated AIFM1 (∼57 kDa), which was not dependent on caspase Enzymes induce apoptosis, overexpression of full-length AIFM1 can induce caspase-dependent apoptosis and inhibit cell growth of liver cancer cells, our data reveal the potential role of rAd-AIFM1 in HCC gene therapy (31). The gene discussed is AIFM1; the disease is liver cancer.