Intriguingly, high methylation levels of PTTG1, as well as its potential transcriptional targets (i.e., CHEK2, OCIAD2, UBE2L3, and ZNF367), could predict the poor prognosis of BLCA patients preferably (P < 0.05) (Figs. S4–S7). Here, CHEK2 is linked to bladder transitional cell carcinoma.