They also repressed the differentiation of naïve CD4+ T cells into regulatory T cells in vitro.298 In a xenograft tumor model of HCC, TN-CAR NK-92 cells reduced tumor load and show increased infiltration into tumor because TN-CAR increased the surface expression of CCR3, CCR6, CXCR4, and CX3CR1 on the NK-92 cells.298 This evidence suggests that CAR expression may affect the profiles of chemokine receptors on NK cells, which should be considered when deciding which types of cancer to target in vivo. This evidence concerns the gene CD4 and neoplasm.