For this, we performed RNA sequencing (RNA-seq) of the immortalized K562 myeloid leukemia cell line following depletion of DHX34, which was verified by qRT-PCR (Supplemental Fig. S4A,B) and changes in gene expression (DGE) and in alternative splicing were assessed (Fig. 4A–C; Supplemental Fig. S4C,D). This evidence concerns the gene DHX34 and myeloid leukemia.