Subsequently, we detected OTUD5 expression levels in human bronchial epithelioid cells 16HBE and NSCLCs (A549, NCI-H292, NCI-H460, and PC9) using qRT-PCR and Western blot analysis, the results of which revealed that OTUD5 expression levels in NSCLC cells were significantly lower compared to those in 16HBE cells (p < 0.05, Figure 1B and C). Here, OTUD5 is linked to non-small cell lung carcinoma.