Hence, we silenced miR-652-3p expression in A549 cells with a miR-652-3p-inhibitor and uncovered that silencing miR-652-3p led to a reduction in NSCLC cell viability, migration, and invasion, along with increased OTUD5 and PTEN protein levels and diminished ubiquitination levels. This evidence concerns the gene OTUD5 and non-small cell lung carcinoma.